Functional Analysis of the 5′ Genomic Sequence of a Bovine Norovirus
Identifieur interne : 001592 ( Main/Exploration ); précédent : 001591; suivant : 001593Functional Analysis of the 5′ Genomic Sequence of a Bovine Norovirus
Auteurs : Omar Salim ; Ian N. Clarke ; Paul R. LambdenSource :
- PLoS ONE [ 1932-6203 ] ; 2008.
Abstract
Jena Virus (JV), a bovine Norovirus, causes enteric disease in cattle and represents a potential model for the study of enteric norovirus infection and pathogenesis. The positive sense RNA genome of JV is organised into ORF1 (non-structural proteins), ORF2 (major capsid protein) and ORF3 (minor capsid protein). The lack of a cell culture system for studying JV replication has meant that work to date has relied upon
Only two of the three major ORF1 proteins were identified (p110 and 2C) following
The
Url:
DOI: 10.1371/journal.pone.0002169
PubMed: 18478070
PubMed Central: 2364642
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Jena Virus (JV), a bovine Norovirus, causes enteric disease in cattle and represents a potential model for the study of enteric norovirus infection and pathogenesis. The positive sense RNA genome of JV is organised into ORF1 (non-structural proteins), ORF2 (major capsid protein) and ORF3 (minor capsid protein). The lack of a cell culture system for studying JV replication has meant that work to date has relied upon <italic>in vitro</italic>
systems to study non-structural protein synthesis and processing.</p>
</sec>
<sec><title>Principal Findings</title>
<p>Only two of the three major ORF1 proteins were identified (p110 and 2C) following <italic>in vitro</italic>
translation of JV RNA, the N-term protein was not detected. The N-term encoding genomic sequence (5′GS) was tested for IRES-like function in a bi-cistronic system and displayed no evidence of IRES-like activity. The site of translation initiation in JV was determined to be at the predicted nucleotide 22. Following the insertion of an epitope within the 5′GS the JV N-term protein was identified <italic>in vitro</italic>
and within RNA transfected cells.</p>
</sec>
<sec><title>Conclusions</title>
<p>The <italic>in vitro</italic>
transcription/translation system is currently the best system for analysing protein synthesis and processing in JV. Unlike similarly studied human noroviruses JV initially did not appear to express the N-terminal protein, presenting the possibility that the encoding RNA sequence had a regulatory function, most likely involved in translation initiation in an IRES-like manner. This was not the case and, following determination of the site of translation initiation the N-term protein was detected using an epitope tag, both <italic>in vitro</italic>
and <italic>in vivo</italic>
. Although slightly larger than predicted the N-term protein was detected in a processed form <italic>in vivo</italic>
, thus not only demonstrating initial translation of the ORF1 polyprotein but also activity of the viral protease. These findings indicate that the block to noroviral replication in cultured cells lies elsewhere.</p>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gunther, H" uniqKey="Gunther H">H Günther</name>
</author>
<author><name sortKey="Otto, P" uniqKey="Otto P">P Otto</name>
</author>
<author><name sortKey="Heilman, P" uniqKey="Heilman P">P Heilman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gunther, H" uniqKey="Gunther H">H Günther</name>
</author>
<author><name sortKey="Otto, P" uniqKey="Otto P">P Otto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oliver, Sl" uniqKey="Oliver S">SL Oliver</name>
</author>
<author><name sortKey="Asobayire, E" uniqKey="Asobayire E">E Asobayire</name>
</author>
<author><name sortKey="Charpilienne, A" uniqKey="Charpilienne A">A Charpilienne</name>
</author>
<author><name sortKey="Cohen, J" uniqKey="Cohen J">J Cohen</name>
</author>
<author><name sortKey="Bridger, Jc" uniqKey="Bridger J">JC Bridger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oliver, Sl" uniqKey="Oliver S">SL Oliver</name>
</author>
<author><name sortKey="Batten, Ca" uniqKey="Batten C">CA Batten</name>
</author>
<author><name sortKey="Deng, Y" uniqKey="Deng Y">Y Deng</name>
</author>
<author><name sortKey="Elschner, M" uniqKey="Elschner M">M Elschner</name>
</author>
<author><name sortKey="Otto, P" uniqKey="Otto P">P Otto</name>
</author>
<author><name sortKey="Charpilienne, A" uniqKey="Charpilienne A">A Charpilienne</name>
</author>
<author><name sortKey="Clarke, In" uniqKey="Clarke I">IN Clarke</name>
</author>
<author><name sortKey="Bridger, Jc" uniqKey="Bridger J">JC Bridger</name>
</author>
<author><name sortKey="Lambden, Pr" uniqKey="Lambden P">PR Lambden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bridger, Jc" uniqKey="Bridger J">JC Bridger</name>
</author>
<author><name sortKey="Hall, Ga" uniqKey="Hall G">GA Hall</name>
</author>
<author><name sortKey="Brown, Jf" uniqKey="Brown J">JF Brown</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pelosi, E" uniqKey="Pelosi E">E Pelosi</name>
</author>
<author><name sortKey="Lambden, Pr" uniqKey="Lambden P">PR Lambden</name>
</author>
<author><name sortKey="Caul, Eo" uniqKey="Caul E">EO Caul</name>
</author>
<author><name sortKey="Liu, Bl" uniqKey="Liu B">BL Liu</name>
</author>
<author><name sortKey="Dingle, Ke" uniqKey="Dingle K">KE Dingle</name>
</author>
<author><name sortKey="Deng, Y" uniqKey="Deng Y">Y Deng</name>
</author>
<author><name sortKey="Clarke, In" uniqKey="Clarke I">IN Clarke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Bl" uniqKey="Liu B">BL Liu</name>
</author>
<author><name sortKey="Lambden, Pr" uniqKey="Lambden P">PR Lambden</name>
</author>
<author><name sortKey="Gunther, H" uniqKey="Gunther H">H Günther</name>
</author>
<author><name sortKey="Otto, P" uniqKey="Otto P">P Otto</name>
</author>
<author><name sortKey="Elschner, M" uniqKey="Elschner M">M Elschner</name>
</author>
<author><name sortKey="Clarke, In" uniqKey="Clarke I">IN Clarke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glass, Pj" uniqKey="Glass P">PJ Glass</name>
</author>
<author><name sortKey="Zeng, Cq" uniqKey="Zeng C">CQ Zeng</name>
</author>
<author><name sortKey="Estes, Mk" uniqKey="Estes M">MK Estes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sosnovtsev, Sv" uniqKey="Sosnovtsev S">SV Sosnovtsev</name>
</author>
<author><name sortKey="Garfield, M" uniqKey="Garfield M">M Garfield</name>
</author>
<author><name sortKey="Green, Ky" uniqKey="Green K">KY Green</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyers, G" uniqKey="Meyers G">G Meyers</name>
</author>
<author><name sortKey="Wirblich, C" uniqKey="Wirblich C">C Wirblich</name>
</author>
<author><name sortKey="Thiel, H J" uniqKey="Thiel H">H-J Thiel</name>
</author>
<author><name sortKey="Thumfart, Jo" uniqKey="Thumfart J">JO Thumfart</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Clarke, In" uniqKey="Clarke I">IN Clarke</name>
</author>
<author><name sortKey="Lambden, Pr" uniqKey="Lambden P">PR Lambden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sosnovtsev, Sv" uniqKey="Sosnovtsev S">SV Sosnovtsev</name>
</author>
<author><name sortKey="Belliot, G" uniqKey="Belliot G">G Belliot</name>
</author>
<author><name sortKey="Chang, Ko" uniqKey="Chang K">KO Chang</name>
</author>
<author><name sortKey="Prikhodko, Vg" uniqKey="Prikhodko V">VG Prikhodko</name>
</author>
<author><name sortKey="Thackray, Lb" uniqKey="Thackray L">LB Thackray</name>
</author>
<author><name sortKey="Wobus, Ce" uniqKey="Wobus C">CE Wobus</name>
</author>
<author><name sortKey="Karst, Sm" uniqKey="Karst S">SM Karst</name>
</author>
<author><name sortKey="Virgin, Hw" uniqKey="Virgin H">HW Virgin</name>
</author>
<author><name sortKey="Green, Ky" uniqKey="Green K">KY Green</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hardy, Me" uniqKey="Hardy M">ME Hardy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seah, El" uniqKey="Seah E">EL Seah</name>
</author>
<author><name sortKey="Marshall, Ja" uniqKey="Marshall J">JA Marshall</name>
</author>
<author><name sortKey="Wright, Pj" uniqKey="Wright P">PJ Wright</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fernandez Vega, V" uniqKey="Fernandez Vega V">V Fernandez-Vega</name>
</author>
<author><name sortKey="Sosnovtsev, Sv" uniqKey="Sosnovtsev S">SV Sosnovtsev</name>
</author>
<author><name sortKey="Belliot, G" uniqKey="Belliot G">G Belliot</name>
</author>
<author><name sortKey="King, Ad" uniqKey="King A">AD King</name>
</author>
<author><name sortKey="Mitra, T" uniqKey="Mitra T">T Mitra</name>
</author>
<author><name sortKey="Gorbalenya, A" uniqKey="Gorbalenya A">A Gorbalenya</name>
</author>
<author><name sortKey="Green, Ky" uniqKey="Green K">KY Green</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ettayebi, K" uniqKey="Ettayebi K">K Ettayebi</name>
</author>
<author><name sortKey="Hardy, Me" uniqKey="Hardy M">ME Hardy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seah, El" uniqKey="Seah E">EL Seah</name>
</author>
<author><name sortKey="Marshall, Ja" uniqKey="Marshall J">JA Marshall</name>
</author>
<author><name sortKey="Wright, Pj" uniqKey="Wright P">PJ Wright</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jiang, X" uniqKey="Jiang X">X Jiang</name>
</author>
<author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name>
</author>
<author><name sortKey="Wang, K" uniqKey="Wang K">K Wang</name>
</author>
<author><name sortKey="Estes, Mk" uniqKey="Estes M">MK Estes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutierrez Escolano, Al" uniqKey="Gutierrez Escolano A">AL Gutierrez-Escolano</name>
</author>
<author><name sortKey="Vazquez Ochoa, M" uniqKey="Vazquez Ochoa M">M Vazquez-Ochoa</name>
</author>
<author><name sortKey="Escobar Herrera, J" uniqKey="Escobar Herrera J">J Escobar-Herrera</name>
</author>
<author><name sortKey="Hernandez Acosta, J" uniqKey="Hernandez Acosta J">J Hernandez-Acosta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blyn, Lb" uniqKey="Blyn L">LB Blyn</name>
</author>
<author><name sortKey="Towner, Js" uniqKey="Towner J">JS Towner</name>
</author>
<author><name sortKey="Semler, Bl" uniqKey="Semler B">BL Semler</name>
</author>
<author><name sortKey="Ehrenfeld, E" uniqKey="Ehrenfeld E">E Ehrenfeld</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haller, Aa" uniqKey="Haller A">AA Haller</name>
</author>
<author><name sortKey="Semler, Bl" uniqKey="Semler B">BL Semler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaminski, A" uniqKey="Kaminski A">A Kaminski</name>
</author>
<author><name sortKey="Hunt, Sl" uniqKey="Hunt S">SL Hunt</name>
</author>
<author><name sortKey="Patton, Jg" uniqKey="Patton J">JG Patton</name>
</author>
<author><name sortKey="Jackson, Rj" uniqKey="Jackson R">RJ Jackson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meerovitch, K" uniqKey="Meerovitch K">K Meerovitch</name>
</author>
<author><name sortKey="Svitkin, Yv" uniqKey="Svitkin Y">YV Svitkin</name>
</author>
<author><name sortKey="Lee, Hs" uniqKey="Lee H">HS Lee</name>
</author>
<author><name sortKey="Lejbkowicz, F" uniqKey="Lejbkowicz F">F Lejbkowicz</name>
</author>
<author><name sortKey="Kenan, Dj" uniqKey="Kenan D">DJ Kenan</name>
</author>
<author><name sortKey="Chan, Ekl" uniqKey="Chan E">EKL Chan</name>
</author>
<author><name sortKey="Agol, Vi" uniqKey="Agol V">VI Agol</name>
</author>
<author><name sortKey="Keene, Jd" uniqKey="Keene J">JD Keene</name>
</author>
<author><name sortKey="Sonenberg, N" uniqKey="Sonenberg N">N Sonenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutierrez Escolano, Al" uniqKey="Gutierrez Escolano A">AL Gutierrez-Escolano</name>
</author>
<author><name sortKey="Brito, Zu" uniqKey="Brito Z">ZU Brito</name>
</author>
<author><name sortKey="Del Angel, Rm" uniqKey="Del Angel R">RM del Angel</name>
</author>
<author><name sortKey="Jiang, X" uniqKey="Jiang X">X Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Daughenbaugh, Kf" uniqKey="Daughenbaugh K">KF Daughenbaugh</name>
</author>
<author><name sortKey="Fraser, Cs" uniqKey="Fraser C">CS Fraser</name>
</author>
<author><name sortKey="Hershey, Jwb" uniqKey="Hershey J">JWB Hershey</name>
</author>
<author><name sortKey="Hardy, Me" uniqKey="Hardy M">ME Hardy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Daughenbaugh, Kf" uniqKey="Daughenbaugh K">KF Daughenbaugh</name>
</author>
<author><name sortKey="Wobus, Ce" uniqKey="Wobus C">CE Wobus</name>
</author>
<author><name sortKey="Hardy, Me" uniqKey="Hardy M">ME Hardy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kozak, M" uniqKey="Kozak M">M Kozak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Bl" uniqKey="Liu B">BL Liu</name>
</author>
<author><name sortKey="Clarke, In" uniqKey="Clarke I">IN Clarke</name>
</author>
<author><name sortKey="Lambden, Pr" uniqKey="Lambden P">PR Lambden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Southern, Ja" uniqKey="Southern J">JA Southern</name>
</author>
<author><name sortKey="Young, Df" uniqKey="Young D">DF Young</name>
</author>
<author><name sortKey="Heaney, F" uniqKey="Heaney F">F Heaney</name>
</author>
<author><name sortKey="Baumgartner, Wk" uniqKey="Baumgartner W">WK Baumgartner</name>
</author>
<author><name sortKey="Randall, Re" uniqKey="Randall R">RE Randall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Love, Dn" uniqKey="Love D">DN Love</name>
</author>
<author><name sortKey="Sabine, M" uniqKey="Sabine M">M Sabine</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopman, Ba" uniqKey="Lopman B">BA Lopman</name>
</author>
<author><name sortKey="Reacher, Mh" uniqKey="Reacher M">MH Reacher</name>
</author>
<author><name sortKey="Van Duijnhoven, Y" uniqKey="Van Duijnhoven Y">Y van Duijnhoven</name>
</author>
<author><name sortKey="Hanon, Fx" uniqKey="Hanon F">FX Hanon</name>
</author>
<author><name sortKey="Brown, D" uniqKey="Brown D">D Brown</name>
</author>
<author><name sortKey="Koopmans, M" uniqKey="Koopmans M">M Koopmans</name>
</author>
</analytic>
</biblStruct>
</listBibl>
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